A new gene for
flower color (crw, cream wings)
in Pisum sativum L. located on LG I
Święcicki, W.K., Gawłowska, M. and Nawrot, Cz. Inst. of Plant Genet.
Polish Acad. of Sci.,
A line with tolerance to Aphanomyces
euteiches was obtained in 1998 from Dr. Earl Gritton (
The line Wt 11166 was crossed to Wt 3527 with normal white color of sail, wing and keel. The F1 plants had white flowers. Among 128 F2 plants 96 were white flowering and 32 showed cream wings.
The line Wt 11166 was crossed to testerlines with gene markers for different linkage groups. An inheritance of the new character was confirmed. Data in the Table 1 show clear monohybrid segregation with recessive nature of cream wings even though some populations are not particularly large. The reason is the A genotype of some testerline parents resulting in ¼ of total F2 population with the a-allele on which cream wings can be observed (see tables 1, 2 and 3). Gene symbol crw is suggested.
Table 1. Monohybrid segregation for the investigated gene cream wings and gene markers in the linkage group I in F2 populations of the linkage test cross Wt 11166 x : 11238 (K.2296), 11540 (K.2423), 11288 (K.2425), 15860 (K.2559) 11777 (K.2558), 11909 (K.2720), 10357 (K.2796), 11143 (K.2799) 10488 (K.2797), Id 860 (K.2800)
|
Cross |
Gene |
Dom |
rec |
Total |
chi square |
|
K.2296 |
crw |
33 |
10 |
40 |
0.07 |
|
K.2423 |
crw |
43 |
11 |
54 |
0.62 |
|
K.2425 |
crw |
42 |
7 |
49 |
3.00 |
|
K.2559 |
crw |
27 |
9 |
36 |
0.00 |
|
K.2558 |
crw |
56 |
22 |
78 |
0.43 |
|
K.2720 |
crw |
113 |
56 |
169 |
5.97 |
|
K.2796 |
crw |
20 |
7 |
27 |
0.01 |
|
K.2799 |
crw |
83 |
35 |
118 |
1.37 |
|
K.2797 |
crw |
18 |
4 |
22 |
0.54 |
|
K.2800 |
crw |
56 |
19 |
75 |
0.00 |
|
|
Am1 |
111 |
41 |
152 |
0.32 |
|
|
Aero |
136 |
53 |
189 |
0.93 |
|
|
I |
152 |
34 |
186 |
4.48 |
|
|
A |
152 |
37 |
189 |
2.96 |
|
Table 2. Distribution of phenotypes in F2 populations (Wt 11166 x testerlines) and the linkage test for the gene crw |
||||||||||
|
Testerline |
Pair of genes |
DD |
Dr |
rD |
rr |
Total |
Joint chi square |
Cr-O(±SE) (per cent) |
Phase |
|
|
Wt 11238 |
A- Crw |
1 |
1 |
33 |
10 |
43 |
2.0 |
66.4 |
±13.3 |
C |
|
|
K-Crw |
31 |
10 |
1 |
1 |
41 |
1.0 |
65.1 |
±9.6 |
R |
|
|
Wb-Crw |
29 |
9 |
4 |
1 |
43 |
0.0 |
46.9 |
±11.8 |
R |
|
|
Tl-Crw |
28 |
9 |
5 |
1 |
43 |
0.0 |
43.3 |
±12.2 |
R |
|
|
Cp-Crw |
25 |
9 |
8 |
1 |
43 |
0.8 |
35.4 |
±13.2 |
R |
|
|
Te-Crw |
24 |
9 |
9 |
1 |
43 |
0.8 |
33.3 |
±13.4 |
R |
|
|
Gp-Crw |
25 |
9 |
8 |
1 |
43 |
0.8 |
35.4 |
±13.2 |
R |
|
|
S-Crw |
30 |
9 |
3 |
1 |
43 |
0.0 |
51.5 |
±11.2 |
R |
|
Wt 11540 |
Crw-Gp |
29 |
14 |
11 |
1 |
54 |
5.2 |
27.8 |
±12.4 |
R |
|
|
Crw-Wb |
35 |
8 |
9 |
2 |
54 |
0.0 |
49.6 |
±10.2 |
R |
|
|
Crw-A |
2 |
40 |
1 |
11 |
53 |
1.0 |
58.4 |
±11.2 |
C |
|
|
Crw-Aat2 |
20 |
7 |
3 |
2 |
32 |
1.4 |
58.9 |
±11.9 |
R |
|
|
Crw-Lap1 |
21 |
6 |
4 |
1 |
32 |
0.0 |
48.1 |
±13.5 |
R |
|
|
Crw-Pgm1 |
18 |
9 |
5 |
1 |
32 |
1.4 |
37.2 |
±15.0 |
R |
|
|
Crw-Pgm2 |
17 |
10 |
3 |
2 |
32 |
0.0 |
48.2 |
±13.0 |
C |
|
|
Crw-Aldo |
19 |
8 |
3 |
2 |
32 |
0.0 |
56.4 |
±12.3 |
R |
|
|
Crw-Skdh |
18 |
9 |
4 |
1 |
32 |
1.0 |
40.3 |
±14.6 |
R |
|
|
Crw-Acp1 |
7 |
5 |
2 |
2 |
16 |
0.0 |
45.3 |
±17.6 |
C |
|
|
Crw-Pgdp |
18 |
8 |
5 |
1 |
31 |
1.4 |
38.8 |
±15.0 |
R |
|
Wt 11288 |
Crw-A |
1 |
42 |
1 |
7 |
49 |
2.0 |
73.6 |
±13.1 |
C |
|
|
Crw-St |
35 |
7 |
6 |
1 |
49 |
0.0 |
47.4 |
±11.0 |
R |
|
Wt 15860 |
Crw-Creep |
24 |
3 |
8 |
1 |
36 |
0.0 |
50.0 |
±12.5 |
R |
|
|
Crw-A |
1 |
26 |
1 |
9 |
35 |
2.0 |
64.7 |
±14.6 |
C |
|
Wt 11777 |
Crw-Arg |
44 |
12 |
17 |
5 |
78 |
0.0 |
48.9 |
±8.4 |
C |
|
|
Crw-Art |
45 |
11 |
18 |
4 |
78 |
0.0 |
48.6 |
±8.6 |
R |
|
|
Crw-Wlo |
44 |
12 |
19 |
3 |
78 |
0.4 |
42.3 |
±9.2 |
R |
|
|
Crw-Pl |
41 |
14 |
18 |
4 |
77 |
0.4 |
56.0 |
±9.1 |
C |
|
Wt 11909 |
Crw-Def |
87 |
25 |
40 |
13 |
165 |
0.2 |
51.7 |
±5.7 |
R |
|
|
Crw-Chi5 |
80 |
17 |
44 |
9 |
150 |
0.0 |
49.4 |
±6.2 |
R |
|
|
Crw-Wa |
81 |
21 |
45 |
9 |
156 |
0.2 |
46.3 |
±6.2 |
R |
|
|
Crw-Pgdp |
80 |
32 |
41 |
14 |
167 |
0.1 |
47.7 |
±5.9 |
R |
|
|
Crw-Skdh |
90 |
20 |
40 |
12 |
162 |
0.7 |
54.2 |
±5.6 |
R |
|
Wt 10357 |
Crw-Tac |
16 |
4 |
6 |
1 |
27 |
0.0 |
44.3 |
±15.3 |
R |
|
|
Crw-Pet |
17 |
3 |
5 |
2 |
27 |
1.5 |
61.2 |
±12.5 |
R |
|
Wt 11143 |
Crw-N |
54 |
29 |
25 |
8 |
116 |
1.7 |
42.7 |
±7.5 |
R |
|
|
Crw-Fa |
71 |
12 |
26 |
8 |
117 |
1.2 |
58.3 |
±6.3 |
R |
|
|
Crw-V |
68 |
15 |
23 |
10 |
116 |
2.3 |
59.3 |
±6.2 |
R |
|
Wt 10488 |
Crw-Fa |
17 |
0 |
3 |
1 |
21 |
2.3 |
72.0 |
±11.9 |
R |
|
|
Crw-Led |
15 |
3 |
3 |
1 |
22 |
0.0 |
57.1 |
±14.7 |
R |
|
|
Crw-N |
13 |
3 |
3 |
1 |
20 |
0.0 |
55.1 |
±15.8 |
R |
|
Table 3. Distribution of phenotypes in F2 population (Wt 11166. crw x Id 860. am1 aero I ; K.2800) and the linkage test for the gene crw |
|||||||||
|
|
Phenotype |
|
|||||||
|
Gene pair |
Phase |
DD |
Dr |
rD |
rr |
Total |
Joint chi square |
Cr-O ±SE (per cent) |
|
Am1-Crw |
R |
1 |
0 |
28 |
9 |
38 |
1 |
34.3 |
14.1 |
|
Am1-Aero |
C |
100 |
11 |
12 |
29 |
152 |
55.4 |
16.0 |
3.3 |
|
Am1-I |
R |
82 |
27 |
40 |
0 |
149 |
11.5 |
18.6 |
7.8 |
|
Crw-Aero |
R |
22 |
34 |
15 |
4 |
75 |
10.2 |
26.8 |
10.6 |
|
Crw-I |
C |
54 |
1 |
13 |
6 |
74 |
12.5 |
15.2 |
4.6 |
|
Aero-I |
R |
100 |
34 |
52 |
0 |
186 |
17.4 |
16.2 |
7.1 |
Linkage test showed that in most of F2 populations Wt 11166 (crw) x testerline deviations from a correct dihybrid segregation crw – gene marker were not found (Table 2). An exception was K. 2800 (Wt 11166 x Id 860) segregating for crw and A am1 aero I (Table 1). Id 860 is the IPG PAS selection with above mentioned markers of LG I. Dihybrid segregation was disturbed for all gene pairs (joint chi square from 10.2 to 55.4) except for Am1 – Crw. Data for this gene pair are “artificial” as am1 is hypostatic to a and crw to A. Data in the Table 3 suggests the following locus order: Crw – I– Aero – Am1. This is in agreement with the current Pisum map (1) and provides LG I with a new morphological marker below i. A confirming linkage test should include the af marker.
1. Weeden, N.F., Ellis, T.H.N., Timmerman-Vaughan, G.M., Święcicki, W.K., Rozov, S.M. and Berdnikov, V.A. 1998. Pisum Genetics 30: 1-4.