Map position of the locus Ins2

 

Berdnikov, V.A. and Gorel, F.L.             Inst. of Cytology and Genetics, Russian Academy of Sciences

                                                                                                                         Novosibirsk, Russia

 

      The gene Ins2 participates in the formation of a compound leaf, acting as a synergist of the gene Af and an antagonist of the gene Uni (1). The mutation ins2, usually produces incisions on leaflet tips, with their central veins continuing as tendrils.  However the mutation exhibits varying expression and dominance, depending on the genetic background [here this mutation is printed in lower case although it often displays dominance (1)].  The varying degree of dominance of ins2 complicates its mapping in most crosses, but heterozygotes can usually be recognized at the stage of flowering.  In F2 of a cross of our line AFD (1), homozygous for ins2, and the multi-marker line WL851, the mutation ins2 behaved as dominant and showed linkage with some markers of linkage group II. Segregation of phenotypes is shown in Table 1.

 

Table 1. Segregation for the genes of the Linkage Group II in F2 progeny of the cross AFD (Wb, K, ins2, His71, His(2-6)1123) x WL851 (wb, k, Ins2, His72, His(2-6)1221 ).

 

Gene pair

Number of progeny with phenotype1

Joint

Chi-sq.

Recomb. frac.

Stan.

Error

A/B

A/h

A/b

h/B

h/h

h/b

a/B

a/h

a/b

ins2-His(2-6)

21

36

19

--

--

--

2

15

7

3.9

41.8

6.0

ins2-His7

19

49

8

--

--

--

1

14

9

11.9*

27.4

5.1

ins2-wb

71

--

7

--

--

--

5

--

10

13.2**

27.1

5.3

ins2-k

65

--

9

--

--

--

8

--

16

28.3***

19.2

4.5

His(2-6)-His7

7

17

2

9

36

6

1

10

12

21.2**

29.3

4.0

wb-His(2-6)

22

42

19

--

--

--

1

9

7

4.4

36.9

5.8

k-His(2-6)

20

36

16

--

--

--

3

12

9

3.4

38.7

6.0

wb-His7

20

51

12

--

--

--

0

12

5

6.1

31.0

5.4

k-His7

20

44

8

--

--

--

0

17

7

10.7*

29.6

5.4

wb-k

72

--

10

--

--

--

1

--

15

46.9***

12.8

3.7

                           

1Designations:  - A,a - first gene; B, b - second gene; h - heterozygous; capital letters refer to alleles from the first parent. ins2, wb, k are in coupling phase.

*,**,*** - probabilities less than 0.01, 0.001 and 0.0001 respectively.

All calculations were done by the maximum likelihood method with the use of the program ‘Cros’

 

These data allow us to construct the following map, in which distances are expressed in crossing-over percentage.

 

 

 

Acknowledgement: This work was supported by the Russian State Program 'Russian Fund for Fundamental Research', grant No 99-04-49970.

 

1.         Berdnikov, V.A., Gorel, F.L., Bogdanova, V.S. and Kosterin, O.E.  2000.  Pisum Genetics 32: 9-12.

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