PNL Volume 17 1985

RESEARCH REPORTS 15

BSG-STAINTNG ARGUMENTS FOR A SHIFT IN LOGATION OF LINKAGE GROUPS II

AND IV

Folkeson, Donald Institute of Genetics, University of Lund

Solvegatan, Lund, Sweden

Because of the appearance of C-bands in the proximity of the secon-

dary constrictions, BSG-staining, as demonstrated by Lamm (3), has become

a powerful tool for identifying the satellite chromosomes 4 and 7 in

mitosis as well as in meiosis. This method has recently been used by me

(2) to verify the interchanges of L-lll T(3S-7S) and L-112 T(5L-7S).

Further analysis has been carried out on L-108, L-114, and L-180, belon-

ging to Lamm and Miravalle's tester set (6), and L-58, analyzed by Blixt

(1), for the coordination of linkage group IV with a relevant chromosome.

The result of BSG-staining indicates that linkage group II instead

of linkage group IV belongs to the short satellite chromosome viz.

chromosome 4 (see table and photomicrograph).

16 PNL Volume 17 198 5 RESEARCH REPORTS

1. Blixt, S. 1972. Agri Hort. Genet. 30:1-293.

2. Folkeson, D. 1984. Hereditas 101:119-121.

3. Lamm, R. 1981. Hereditas 94:45-52.

4. Lamm, R. 1983. PNL 15:33-35.

5. Lamm, R. 1982. PNL 14:32-35.

6. Lamm, R. and R. J. Miravalle. 1959. Hereditas 15:417-440.

LIGHT

Gaul,

Table 1. Compilation of cytogenetic results involving four

translocation lines.

Key to table:

1. Origin of involved lines

2. Previous assumption of chromosomes involved in the interchanges

3. Linkage groups involved

A. Appearance of C-band in the interchange system (ring of four)

5. Reference

6. Key to photomicrograph

Fig. 1. Giuemsa C-bands in meiosis of pollen mother cells. For

key to figure see Table 1. The arrows indicate the

location of the C-bands; bar represents 10 mkm.